Methylene Blue Method. Absorbance of blank is higher than samples, why?

Muhammad Salman Munir Malik @Muhammad-Salman-Munir-Malik

08 April 2022 0 9K Report

Hi all. I am using methylene blue method to determine Hydrogen sulfide gas. Usually my blank is higher than the samples. For example blank is 0.00 and samples absorbance comes -0.0022,,,, -0,0191 etc. while my expected absorbance is 0.0020-0.0599.

Does the type of cuvettes interfere with lower concentrations? Any suggestions?

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