Dialysis tubing vs Protein concentrators?

I am looking into doing a peptide conjugation and the protocol im looking at says to use dialysis tubing to separate the reacted peptide from unreacted. I do not have much of the peptide so i...

09 October 2019 5,555 3 View

Quick isolation of basic peak on an antibody?

Im working on an antibody that has a strange basic peak that does not come down after CpB treatment. After treatment, the peak averages at 5%. I attached pictures below. Mainly interested in the...

11 December 2018 5,634 2 View

Anyone have a trans-membrane TNF alpha expressing mammalian cell line?

Anyone have a trans-membrane TNF alpha expressing mammalian cell line that you would be willing to share a couple vials of? Just need it for a FACS binding assay. I cant find any for sale online,...

08 September 2018 5,748 1 View

Is there a method to purify small amount of DNA at high concentration?

Hi all, I need to screen a random library of genetic elements in mammalian cells after cloning, so I need to DNA purify >50 constructs. I understand that the concentration of DNA from a...

05 June 2018 1,494 7 View

How to enhance mammalian cell growth rate through media additions?

I need to reduce the length of my cell culture process. I was thinking of doing this by shifting log phase earlier on, but I cant find any papers that have enhanced cell growth through media as of...

04 May 2018 2,610 4 View

Are there any CHO genome databases with genomic data on >10 CHO cell lines?

I just keep finding data on CHOK1, CHOK, DG44, DXB11. I was hoping I could get access to quite a few CHO genomes to make genomic fingerprint comparison easy. My backup plan is to use mouse cell...

03 April 2018 7,287 3 View

Are there any ~1 nm size fluorescent particles?

Or what is the smallest known fluorescent molecule?

11 December 2017 8,321 3 View

How to reduce mammalian cell culture length?

My lab is working with a novel CHO cell line adapted from CHO-K1. We would like to use it for production however, its culture lengths are 20+ days. We tried to reduce culture length by starting...

08 September 2017 6,125 5 View

What is the lowest level life form with highest complexity immune system?

Are there bacteria or fungi with immune systems to attack neighboring foreign cells? Do insects exhibit GVHD (can they distinguish their own cells from another insect of the same species)?

08 September 2017 2,112 8 View

Silanization dryness conditions?

Is 200 proof Molecular biology grade ethanol dry enough for usage with APTES for silanization of glass? And what kind of results would i get if I perform the silanization in a normal chemical hood...

07 August 2017 8,273 3 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View