Dear Marine Biologists,
Do you have any experience in collecting plasma of abalone that you could give me some advice? I see 2 different methods from publication:
· cut adductor muscle in each animal with a scalpel, and collect hemolymph from the blood sinus (Zhou et al., 2015)
· collect from the pedal sinus using a 1 ml syringe with a 27-gauge needle (Venter et al., 2018a).
Which method is better? If we cut the adductor muscle, hemolymph may get contaminated with mucus?
Many thanks.
Thao
Also from: Stegeman, N., Allender, M., Arnold, J. and Bonar, C.J. (2020). Aquatic Invertebrates. In Exotic Animal Laboratory Diagnosis (eds J.J. Heatley and K.E. Russell). doi:10.1002/9781119108610.ch21
"In abalone, hemolymph can also be collected from the branchial
vein with a 5 ml syringe and a 1 in., 20 g. needle [35], or
the pallial sinus using a 1 ml syringe with a 25 g. needle
[2]."
Refs therin:
2. Cheng, W.T., Hsiao, I.S., and Chen, J.C. (2004). Effect of nitrite on immune response of Taiwan abalone Haliotis diversicolor supertexta and its susceptibility to Vibrio parahaemolyticus. Diseases of Aquatic Organisms 60:157–164.
35. Nollens H.H., Keogh J.A., Probert P.K. 2003. Effect of shell lesions on survival, growth, condition, reproductive capacity and hematology of the New Zealand blackfoot abalone, Haliotis iris. Presented at 34th Annual Conference of the International Association of Aquatic Animal Medicine.
Characterization of hemolymph phenoloxidase activity in two ...
parasitesandvectors.biomedcentral.com › articles
by W Le Clec’h - 2016
This link might help you -
https://www.abc.net.au/catalyst/antiviral-abalone/11015406
Hello Thao,
I've worked on abalone plasma few years ago. Plasma sampling is very simple on abalone. All you have to do is to withdrawn hemolymph from the cephalic arterial sinus in the anterior muscle, using a needle (25 G 0.5 × 16 mm) fitted onto a 2 mL sterile syringe. Then centrifuge the collected hemolymph at 785 g for 10 min at 4 °C, to separate the cellular fraction (hemocytes) from the plasma.
http://linkinghub.elsevier.com/retrieve/pii/S0044848613006406
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