Dear

Biuret method can be used to measure  amount of protein but I suggest you confirm it by bradford test.

Thank

Marius

As long as the protein is dissolved in the reagent, you can measure it. If the protein is not dissolved, it will not react.

Also, if there is other undissolved material, it will give a background in the absorbance measurement. Non-protein undissolved material should be removed by centrifugation after the reaction. The supernatant should show no turbidity.

Thank you Marius K. Somda and Adam B Shapiro