I use method of Hou et al. (2003) for Angiotensin I Converting Enzyme inhibitory assay.
Hello I want determine degree of hydrolysis by measure the percentage of protein soluble in 10 % trichloroacetic acid /total protein in sample, how I can measure the total protein with biuret...
11 December 2016 7,783 2 View
what is the best solvent for ACE enzyme
11 December 2016 8,721 0 View
Is it possible that using a biuret method to measure protein of suspension mixture?
10 November 2016 8,503 3 View
This was found in Persian gulf. I thought that second crab is Liagore erythematica and third crab is dorippoides nudipes, are these true?
05 June 2016 4,793 5 View
This was found in Persian gulf.
04 May 2016 6,037 7 View
Hi, to determine the degree of hydrolysis of proteins,I want use the hoyle and merritt method. DH= 10% TCA soluble N in sample/Total N in sample *100 For the determination of total nitrogen in the...
04 May 2016 1,162 0 View
This was found in persian gulf.
03 April 2016 3,936 6 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
I'm interested in learning about enzyme usage. Could someone explain the basics of working with enzymes? I understand they're different from standard chemicals, but I'm uncertain about the...
01 August 2024 5,460 5 View
I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...
30 July 2024 2,483 5 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
I am working on carbonic anhydrase immobilization into MOFs. I am facing problems with low enzyme loading.. The other issue is that when using p-NPA activity test to detect the activity of the...
20 July 2024 1,440 3 View
when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.
17 July 2024 3,532 0 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?
15 July 2024 1,598 3 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View