Hello, I stimulated MCF7 and MDA with LPS. I used Cytokine arrays later on to study the effects of this stimulation on the secreted cytokines.

I discovered that there are no significant difference between the cells stimulated with LPS and the control cells.

I stimulate my cells with 10µg/mL of LPS during 6 hrs and 30 hrs.

Why do you think that the LPS didn't start an inflammatory reaction in these cells?

Thank you for your help