Ralph Nehme

4 Questions 2 Answers 0 Followers

Questions related from Ralph Nehme

the expression of inflammatory genes and the secretion of inflammatory cytokines are not always correlated ?

Hello, I stimulate Cows PBMC with LPS and with an anti-inflammatory (6 hrs before the LPS) and another case (6 hrs after the LPS) Incubation time 24 hrs Results : RT Pcr decrease in the...

23 August 2023 2,005 0 View

MCF7 and MDA stimulation with LPS?

Hello, I stimulated MCF7 and MDA with LPS. I used Cytokine arrays later on to study the effects of this stimulation on the secreted cytokines. I discovered that there are no significant difference...

06 October 2021 1,501 1 View

My PBMC cells form some aggregates that cannot be dissolved in the culture media (RPMI). What should I do?

I did a flux cytometry and i'm sure that my cells are PBMC (from cow) , but i don't understand why after the centrifugation i have a lot of cells stick to each other (cannot be dissolved in the...

19 April 2021 9,215 2 View

The contamination of PBMC with red blood does-it influence negatively the culture of the PBMC?

Hello, I'm doing a PBMC culture to study the effect of essential oils on the cytokines produced by the PBMC (PCR). In my Culture media i detect the presence of red blood. Should I do something to...

04 January 2021 2,733 3 View