Is it possible that in a co-infected host cell, DNA of a recombinant AAV integrates into the DNA of a wild-type adenovirus with non-homologous end-joining? Thus, may the wild-type adenovirus spread the transgene from the AAV to other cells?
It is more likely that homologous recombination will occur either side of the transgene resulting in the integration. Still very possible and quite likely given how prone ssDNA viruses are to homologous recombination.
Thank you Robert! So, if homology is negligible then it is unlikely (or impossible) to recombine with other (non-homologous) methods with distinct type of viruses?
Then there would need to be NHEJ or transposon mediated horizontal gene transfer. As you said those routes are considerably less common.
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