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The Folin-Ciocalteu method is an electron transfer based assay, and gives reducing capacity, which is usually expressed as phenolic content. Can anyone share any reference paper where total...

13 February 2021 3,782 3 View

How does He-O plasma react with PET surface?

I used He-O plasma irradiation to activate the surface of polyester aka PET fabric before coating the fabric with polypyrrole via in-situ polymerization of pyrrole monomer in an oxidative...

22 March 2020 3,327 5 View

Analysing Peak Fitted Spectroscopy Data?

What is the significance of (or how do I interpret) the "offset value (Yo)" while I peak-fit spectroscopy data (say FTIR, UV-Vis, XRD etc.) in Origin 8.0 Pro? I understand a more positive offset...

26 November 2019 5,620 3 View

How can we make the silver electrodes on 1mm crystal ?

About making device of 1 mm crystal.

22 May 2018 1,530 4 View

Can we scale down the dimensions of ASTM(d638) standard specimen? if yes, then how?

I need to prepare a tensile specimen from a sample of diameter 40 mm. It is a composite of PCL-HA-Mg made via cold pressing. Also, suggest the astm d638 type which i should scale down?

08 November 2017 2,059 3 View

How can I make PCL powder from pellets?

20 August 2017 8,172 4 View

Can I use molecular dynamics simulations of friction stir welding?

I am working on Friction Stir Welding, and I want to know How can I use Molecular dynamics for FSW simulation?

26 January 2016 3,052 3 View

Can someone help with nick translation/probe labelling through fish insitu hybridization?

I am trying to label a probe with flurescein dye through nick translation. In the previous step, PCR with the respective primers is yeilding a desired amplicon of 250 bp on agarose gel. But after...

11 May 2015 5,355 7 View

Soil Sampling and Analysis

If the physico-chemical parameters of the soil from a paddy field has to be assessed, what would be the proper sampling procedure? Should I collect the samples from different depths i.e. surface,...

02 May 2013 532 12 View

Thin ceramic coatings

Developed high performance thin ceramic coatings of Cr2O3, Al2O3, Al2O3-TiO2, YSZ Low Ra value 4-5 microns High bond strength Thickness 40 to 100 microns No post treatment is required Can be...

01 January 1970 2,547 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

What is the best method to evaluate and measure non-circulating estrogen levels in mice ?

Could anyone suggest me the best procedure to measure the circulating estrogen levels in mice ? I came across the general methods where it can be measured in serum or urine but a detail suggestion...

22 February 2021 4,352 2 View