Maximum OD value and Curve Fitting in sandwich ELISA?

20 May 2021 1 7K Report

I did an experiment in CA 15-3 tumor marker measurement. I use ELISA and get OD value for standard : 0.00675 0.13325 0.54125 1.44975 1.96425 . Are that still an acceptable value to use as standard curve?

What's the best curve to use as curve fitting? Linear with R squared value = 0,92 or 4PL with R squared value = 1 ?

Seyyed Morteza Hoseini

The best standard graph is one with the highest R2.

In ELISA, it would be curve not line. Plot the obtained absorbance against concentration and calculate R2. If it is high, the graph is good

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