Is that possible to include evidences generated from systematic review and meta-analysis as sub part of PhD dissertation work? How?

Hello there, in the process of conducting PhD dissertation, we generated evidences through systematic review and meta-analysis. Based on that, we conducted the experiments. Is that possible to...

12 December 2019 2,446 14 View

Could I use ELISA kit for MUC 1 to measure the CA 15-3 ?

I currently doing a research for anticancer drugs and have a plan to use CA 15-3 as tumor marker to measure the effectivity. Some reference tell that CA 15-3 and MUC 1 are synonym, but i also...

10 September 2019 2,125 5 View

How TSN over Ethernet can be integrated with AI in order to compute the adequate traffic scheduling for the network?

TSN Ethernet Integration Artificial Intelligence Switches To compute the adequate traffic scheduling For the network?

21 March 2019 3,683 1 View

Autonomous Vehicle: what is the new customers market potential of autonomous cars?

Market like India has strong resistance against autonomous vehicles due to fear of unemployment. What is the potential of Indian market in the context of autonomous vehicles by 2030? what is the...

17 March 2019 2,633 2 View

What is the next most promising alternative of Li-Ion?

There are many alternatives of Li-Ion batteries such as Na-Ion, K-Ion, Hydrogen Fuel Cells, Zinc based RFBs etc. But like lithium what is the most promising alternative source of electric energy...

17 March 2019 9,618 3 View

Hello there, I got a chance to write a book chapter and I have to submit proposal. Can anyone guide me how to write the proposal?

Hello there, I got a chance to write a book chapter and I have to submit proposal. Can anyone guide me how to write the proposal?

30 December 2018 3,267 13 View

Why does aztreonam ineffective against gram-positive bacteria, Is the permeability a reason or poor affinity towards PBP3 of Gram +ive bacteria?

Can anyone explain, Why does aztreonam ineffective against gram-positive bacteria, Is the permeability a reason or poor affinity towards PBP3 of Gram +ive bacteria? please explain.

31 May 2017 6,444 3 View

Why the E-cadherin cis dimers were never observed using direct biophysical methods while these were observed for EC1 domain crystal structure?

The crystal structure of EC1 domain of N-cadherin was determined by Shapiro et al. in 1995. In that structure he observed cis dimers. Although many biochemical methods have provided evidence of...

23 October 2016 3,222 1 View

What is the physiological concentration of E-cadherin?

02 October 2016 9,584 3 View

Why do I get PCR amplification but no release of the fragment when digested with appropriate restriction enzymes?

Has anyone come across a situation when you get a amplified band in PCR, using a recombinant vector as template (tested alongside the positive control; genomic DNA as template) but no release of...

20 July 2015 7,109 4 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View