What is the maximum number of personal articles that an author should cite in his or her new article?

It is a good practice for an author to cite some of his or her published work. However, this practice has been abused to gain citations.

21 December 2020 1,385 8 View

I'm looking for a good pH 13 buffer recipe for hydrolysis experiment?

I did try phosphate using Na2HPO4.7H20 and NaOH but buffering capacity was very low especially upon dilution.

09 August 2020 9,692 4 View

I am faced with obtaining SVD and pseudo inverse of this (3x2) matrix A?

Please can someone help with the SVD of the matrix A and send me the pictures to my email? [email protected] Please note, I have tried the possible ways and I am still working on...

11 August 2019 8,468 3 View

Doubly periodic conditions (two-directions) for turbulent rectangular channel flow using LES in ANSYS Fluent?

I need a periodic BC where mass flow rate is specified to model fully developed flow, and another periodic BC with no pressure drop. However, once I define the mass flow rate for one periodic BC,...

18 November 2018 4,845 7 View

I'm planning a glucose uptake assay.

I'm planning a glucose uptake assay with adult mesenchymal stem cells and possibly rat neural stem cells for my undergraduate thesis. i'd appreciate some recommendations for uptake kits and...

18 July 2017 2,222 1 View

In soil contaminant transport, what are other modes of transport apart from advection, sorption and degradation?

03 February 2017 1,444 4 View

How do I pool RNA samples for sequencing?

We have done a disease model mouse experiment, for which there were two treatments, let's call them A and B, yes/no. So we have 4 strata, and we have 9+/-1 samples per strata. We aim to look...

13 September 2015 7,901 12 View

What pH of refolding buffer will be suitable for refolding (renaturing) a solubilized Inclusion Body of protein with isoelectric point (PI) 6.2?

I am trying to solubilize and refold an inclusion body of NKG2D with theoretical isoelectric point (PI) value of 6.2. I usually purify my solubilized protein with Nickel column (Elution buffer pH...

15 December 2013 4,955 4 View

Can any one help me with a suitable protocol for double digestion of a vector(pET22b) and an insert (DNA) with BAHM1 and NOT1 restriction enzymes?

I want to insert my recombinant DNA of about 1650bp into pET22b which is about 5500bp. The resultant product from the ligation will be used for E. coli transformation. I seem not to get a...

21 September 2013 5,706 12 View

Suitable DNA codon for the linker (G4S)3 for expression in E. coli?

I want to link two proteins with the linker (G4S)3. The linked (fused) proteins will then be expressed in E. coli. I will therefore be grateful if anybody could recommend a suitable DNA codon for...

07 March 2013 3,645 1 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

Can any scholar assist me with an r code for calculating average bias for each parameter of a continuous distribution via simulation?

I want to perform a simulation study to examine the consistency property of parameters of a new distribution through the quantile function. The simulation study will contain many replicates (say...

10 February 2021 180 3 View

Should we analyze or relinquish the clinically confirmed data of COVID-19?

Hello everybody As you know, due to the high cost and time-consuming of laboratory assays, like PCR, for the suspected cases of COVID-19, doing this type of investigation is not possible for all...

06 February 2021 6,122 3 View

Does Location Map of Informal Settlements in South Africa exist?

I urgently need a map that shows the point locations of informal settlements in South Africa. I am currently working on a study that seeks to relate protests location to the location of informal...

02 February 2021 4,113 7 View

Is a Strong correlation between the Moderator variable and the dependent variable an issue for moderation model (model 1) in Hayes PROCESS?

In a typical moderation model with one moderator, is it a problem if the moderator and the dependent variable show a strong correlation (say 0.85)? What if a strong correlation exists between the...

27 January 2021 4,937 4 View

Does the power law behavior can be approximated with Gaussians?

In epidemiology, earthquakes, tokamak disruptions etc., there is possibility of approximations with the sequence of Gaussians and with the appropriate risks ( see my papers in Journal of Fusion...

26 January 2021 5,411 5 View

Any method or procedure of parameters estimation for epidemiological mathematical modeling?

As in the image below, may someone help us on how to estimate the parameters used on epidemiological mathematical modeling described in systems of non linear differential equations? Any possible...

22 January 2021 2,869 3 View