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I have thawed my cells (3T3 Fibroblasts) recently. Why am I seeing black pellet of cells after centrifuging during the subculturing?

I have thawed my cells 3T3 (fibroblasts) a couple days ago. After thawing I added 9ml of warm media into the cells and centrifuged them at 1000rpm for 5minutes. I sub-cultured my cells twice but...

16 January 2024 9,830 2 View

The request of Providing the permission to use and providence of contact information for "Emotional Maturity Scale" in Research?

Anyone knows the functional email contact information of "Emotional Maturity Scale (Singh & Bhargawa, 1990)" where I can seek permission to use it in my current research?

20 August 2023 152 7 View

Can anyone tell about EARLY SELECTION OF PEA GENOTYPES AT SEEDLING CHARACTER UNDER WATER DEFICIT conditions?

I am writing my thesis, I need help in this. only drought condition is applied not salts stress..

27 May 2021 4,800 2 View

Iron nitrate nonahydrate with PEG,PVP and ethane diol convert to iron oxides equation?

Iron nitrate nonahydrate with PEG,PVP and ethane diol convert to iron oxides equation

03 October 2020 8,592 0 View

Detection of hexavalent chromium by colorimetric agent, how we can prepare 1,5 diphenylcarabazide reagent soloution and added into sample soln.??

Kindly provide me guideline for batch adsorption experimenr.

10 September 2020 9,318 3 View

Can methanol be used in place of ethanol in in- situ synthesis of metal ferrite supported g-C3N4 ?

I am working on in- situ synthesis of g-C3N4 supported metal ferrites.. i have to ask that can i used methanol instead of ethanol in synthesis ? Is it suitable for this reaction??

20 August 2020 7,794 3 View

I m looking for a "professional jealousy scale", can anyone help me with the search or share a link to get access to this scale?

A full author detail is required to access the scale.

13 February 2020 2,882 3 View

Which media is good for HUVECs cells?

I have never worked before with HUVECs. Can I use high glucose DMEM with 10%FBS and 1%L-glutamine, Penicillin streptomycin and sodium pyruvate? Is it better to use RPMI?

30 January 2018 8,923 3 View

Does anyone know the assay for quantifying the conjugation of Methacrylic anhydride with alginate?

I am reacting methacrylic anhydride (MA) with alginate. MA react with OH group of alginate. Is there any assay to quantify the modification?

22 August 2017 7,092 1 View

What is the appropriate solvent for EDC/NHS chemistry when COOH containg compound is polar and amine containing compound is non polar?

Actually, i am trying to attach amino anthracene with sodium alginate by using EDC/NHS chemistry. Amino anthracene is non-polar and only dissolve in the organic solvent. I am using phosphate...

08 June 2017 6,250 3 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

Hello Everyone ! I'm looking for a good journal to publish my manuscript with low publication cost?

I am Looking for a Science Journal with good impact factor and low publication cost to publish a review paper. Your suggestions would be appreciated.

06 August 2024 6,796 3 View

Can Marketing stop the War ?

Can marketing stop the war ?

06 August 2024 3,661 11 View

Is an invitation to join the editorial board of Clinical Cardiology Updates a scam?

I received an e-mail invitation to join the editorial board of Clinical Cardiology Updates. While I have published a few articles related to cardiovascular disease, there are lots of colleagues...

06 August 2024 8,981 8 View

Is Galaxy.org good to use for research for analyzing data and for publication?

Hello all, I wanted to know, can I use galaxy (USA, Europe or Australia) platform for analyzing the shotgun data, and can it be used for publication purpose as well? Thanks :)

06 August 2024 6,610 4 View

How to convert a privately loaded document into a public document?

I attempted to make a privately uploaded text public but a window appeared that said an error occurred. There was no explanation provided as to why there was an error or what might be done to...

05 August 2024 8,025 7 View

Why is the Protocol for Preventing Postpartum Hemorrhage (https://pubmed.ncbi.nlm.nih.gov/36113283/ ) not being widely adopted?

If the placenta is delivered in squatting position within 3 minutes of the birth of the baby, postpartum hemorrhage is completely eliminated. It works every time. It is quite likely that if a...

05 August 2024 8,895 0 View

How do i get an account to upload my published papers?

need to open an account to upload my published papers

01 August 2024 9,255 1 View

How to send my account link and how to make my account public?

Nothing

01 August 2024 755 1 View

How can productivity (using the Google form link below to provide your answers) be achieved in manuscript publication?

Survey on Productivity in Journal Manuscript Publication Survey Form Link: (https://forms.gle/YRVrn8dL4WZJJ79S8 ) Dear Researcher, We kindly invite you to participate in our survey focused on...

29 July 2024 4,116 1 View