How do I change g/l into mM for bacto agar. I am trying to get the millimolar content of sodium and chloride percentage in bacto agar. How do I do it?

I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...

09 February 2021 4,158 1 View

Why does the DNA's elution decrease after centrifuging with spin column?

02 January 2021 987 4 View

A three level hierarchical data structure in multilevel modelling, for multiple response question?

Im refering to Andy Field's example in his book Discovering statistics using SPSS, 3rd edition, page 728, where he gives an example of a data structure with level 1 variable is a memory recall...

26 December 2020 9,717 6 View

What is the reasons of increased th molecular weight of some chemicals in plant when the concentration of a pollutant that effectin on plant?

please need paper explain this case

23 December 2020 2,657 2 View

RBC's contamination during DNA extraction ?

Hi there! currently i am working with spin column kit for DNA extraction I've put almost 600 uL of buffy coat. after a lot of washing, the RBC's remained stucking in filter? How could i get rid...

25 October 2020 8,712 4 View

How to measure internal validation of multiple regression using a split-sample method with dichotomous variables?

I would like to validate a regression model using a 50-50 (or 80-20) split method. In my initial multiple regression I have entered all predictor variables using 'enter' technique. My sample size...

20 October 2020 2,849 5 View

How to aggregate expert opinion for Fuzzy AHP methodology?

Any recommended method or approach? Appreciate the support.

11 October 2020 5,290 6 View

Can methanol be used in place of ethanol in in- situ synthesis of metal ferrite supported g-C3N4 ?

I am working on in- situ synthesis of g-C3N4 supported metal ferrites.. i have to ask that can i used methanol instead of ethanol in synthesis ? Is it suitable for this reaction??

20 August 2020 7,569 3 View

What are the psychological effects on children during the COVID 19 pandemic?

I am working on the Psychological impact of COVID 19 on children of health care workers. i need references and guidelines for research on this topic.

20 July 2020 2,791 19 View

I need a protocol for Cas9 expression in E coli?

Hello Dear researchers, I am trying to express Cas9 vector in E coli but finding difficulties. After IPTG induction I am unable to get any protein(Cas9) via His-tag chromatography. Also, there is...

17 June 2020 1,170 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View