Making of 1M potassium phosphate buffer?

12 December 2017 0 8K Report

I need to make 1M potassium phosphate buffer pH 6.51. I have KH2Po4 and k2Hpo4,3H20. Acoording to the calculation , pH=pKa+log salt/acid I added 0.691 M KH2po4 and 0.309 M k2HPO4,3H2O for 100 ml solution. pKa 6.8 was used.

After adding them in 50 ml water I had to use 22 ml of 1M NaOH to bring the pH to 6.51. Rest was filled with water upto 100 ml. Am I doing any mistake? I do not have K2Hpo4(Anhydrous), so I had to use trihydrate.Some of my friends are telling me I do not need to adjust the pH.

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