Magnesium stearate solubility in DMSO?

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What is the best way to quantify the specific antibodies against the venom in my neutralization assay?

I need help with a neutralization assay of chicken IgY incubated with rattlesnake venom. I have hyperimmune chickens that have been vaccinated with venom. I have the purified IgY. I have tried...

07 August 2014 3,437 8 View

Is IgY non-specific and if so how do I overcome the non-specific binding?

I am trying to develop an ELISA that is coated with an antigen and specifically binds IgY. What I am seeing is that a control IgY is non-specifically binding as well. Is IgY non-specific? If so...

03 April 2014 1,676 3 View

Extraction of glycerol from restriction enzymes

What is the best method for extraction of glycerol? Dialysis seems to be lowering the activity of the enzymes.

07 August 2012 6,735 2 View

I have immunoglobulins from canine plasma, but they are contaminated with albumin. What is the best single step to remove albumin?

I do not have access to chromatography. I have read that zinc or copper will precipitate the albumin.

09 October 2011 8,139 6 View

What is saturation solubility of insulin in saliva?? I need it to know sink condition ?

05 August 2024 4,407 0 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

I need to dissolve stigmasterol for an animal experiment, it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent?

Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?

01 August 2024 7,504 2 View

How to explain the different interaction between soluble and insoluble polymer in water?

Hello everyone, I am try to understand the differences interaction between insoluble and soluble polymers in water with metal ions and how theit charge density (of metal ions) affect the interaction.

29 July 2024 6,350 3 View

What is the solubility of Iron (+3) in Bismuth Oxide?

Dear all: I am modifying Bismuth Tungstate with Hematite (Fe2O3), so I would like to know about how much Iron(+3) can form a solid solution in a Bismuth Oxide matrix. Best wishes Marco

29 July 2024 2,163 1 View

How do regenerate Amberlite XAD 4 resin for further cycles of use for removing 0.5% Sarkosyl from recombinant protein solutions?

I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...

25 July 2024 5,001 2 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

Why my gel electrophoresis has smearing?

Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...

22 July 2024 9,761 5 View

What is the suitable solvent of MIL-100 for chemotherapy?

I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...

19 July 2024 8,522 0 View

A twin-shaft kiln or a rotary kiln for lime production 1 class? if compressive strength of limestone is low 60 MPa?

My research work is related to the calcination of limestone. I have a complex question and would like to consult with experts in this field. Could you please advise which kiln to choose (a...

18 July 2024 1,390 2 View