I do not have access to chromatography. I have read that zinc or copper will precipitate the albumin.
You can use G-protein coupled to Sepharose beads. It is an easy and fast, just mix the suspension of protein G-Sepharose with your material then centrifuge and wash. The product label (protein G-Sepharose describes the steps). As you may know the protein G has affinity to IgG that is the most abundant Immunoglobulin in serum.
We used ti separate immunoglobulin from serum this way, with:
The ammonium sulphate globulin precipitation technique. -Manipulations are carried out at room temperature. Equal volumes of mouse serum, usually 50 ul, made up to a volume of 0.1 ml with 0*1 M phosphate buffered saline pH 7.3 in conical tubes used (were of 4 ml capacity, Gelman Hawksley Ltd). After incubation, 0-2 ml saturated (NH4)2SO4 was added to each tube, centrifuging at 15,000 rev/min for 5 minutes in a Beckman 152 Microfuge. The supernatant containing albumin is removed and the precipitate, now immunoglobulin enriched, is washed twice with half-saturated (NH4)2SO4.
Reference
Immunology, 1972, 22, 747.
The use of Ammonium Sulphate Globulin Precipitation
for Determination of Affinity of Anti-Protein Antibodies
in Mouse Serum
M. W. STEWARD AND R. E. PETTY
Thanks for the input, we have eliminated about 95% of any no immunoglobulin fractions.
Pierce has a kit that utilizes the Cibacron Blue method.
Pierce Blue Albumin Removal Kit
The Same advice to you Jeff:
We used to separate immunoglobulin from serum albumin this way:
The ammonium sulphate globulin precipitation technique. -Manipulations are carried out at room temperature. Equal volumes of mouse serum, usually 50 ul, made up to a volume of 0.1 ml with 0*1 M phosphate buffered saline pH 7.3 in conical tubes used (were of 4 ml capacity, Gelman Hawksley Ltd). After incubation, 0-2 ml saturated (NH4)2SO4 was added to each tube, centrifuging at 15,000 rev/min for 5 minutes in a Beckman 152 Microfuge. The supernatant containing albumin is removed and the precipitate, now immunoglobulin enriched, is washed twice with half-saturated (NH4)2SO4.
Reference
Immunology, 1972, 22, 747.
The use of Ammonium Sulphate Globulin Precipitation
for Determination of Affinity of Anti-Protein Antibodies
in Mouse Serum
M. W. STEWARD AND R. E. PETTY
Here is the article which can help you in separating immunoglobulin from serum albumin. Let me know if you have any specific question.
Article Discriminatory protein binding by a library of 96 new affini...
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