What is the best method for extraction of glycerol? Dialysis seems to be lowering the activity of the enzymes.
Hey,
I cant understand why you want to get rid of glycerol present in the restriction enzyme buffer. My simple idea is, if possible, dilute it with the same buffer which doesnt have glycerol and concnetrate the same with the small cut-off columns.
Hope it may help.
It would be helpful to know what you need the DNA for. If it's another enzyme reaction, you could dilute it as Ananda says, and either then concentrate it or not. You could also ethanol precipitate it. I often use a Qiagen column to purify the DNA. Note that people often use more restriction enzyme than they need to. If you minimize the amount added, you will be adding less glycerol. Also, many of the REs are hardy, and will remain active for a long time at 37C. So you can add a fraction of the standard amount and then let the reaction run longer.
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