I am using Lymphoprep from Steamcell but even following my protocol as I used with Hystopaque, my PMBC has a lot of platelets.
Someone has observed the same?
Help me please
I am trying with buffy coat, fresh blood.
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
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Hi all, My lab has Thermo Scientific™ Invitrogen™ EVOS™ FL Auto 2 Imaging System, and I was wondering if I will be able to use it with whole blood, whilst focusing on platelets? The idea would...
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We were imaging platelets under SEM. We got this structure. Any recommendation on what this structure could be will be of great help. Thank you
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What is the cause of increasing only WBCs while RBCs decrease although it is the same line in erythropoiesis
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Hello everyone, I need help to understand how I can "co-cultivate" human platelets (derived from whole blood) with cancer cells. I have specific questions: -How can I avoid platelets from...
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Red bone marrow contains blood stem cells that can become red blood cells, white blood cells, or platelets. Yellow bone marrow is made mostly of fat and contains stem cells that can become...
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Does the presence of RBCs decrease total fibrinogen concentration in an assay that has to be performed in whole blood? In vitro experiments were performed in isolated platelets in which fibrinogen...
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Hello everyone, I would like to use platelets in a co-culture with other cells. I was wondering which are the conditions to keep alive the platelets: -since I am going to buy my platelets, I was...
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According to the Kit instruction, I'm using 10 to the power 6 cells (platelets). But the no. of events while recording the reading doesn't even cross 10. Kindly suggest me a solution...
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