Why in myloid leukemia the wbcs increase in number while Rbcs and Platelets decrease in number ?

More Hayder Abdullah K. Al-Sarray's questions See All

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

Is there a fast method matching citations to bibliography (manually typed references)?

I need to be able to match in-text citations to a reference list and back again in very large documents (100+ pages) WITHOUT the use of referencing software like Endnote. my method is to type the...

27 February 2021 9,848 1 View

Can I put a figure that was drawn by Bio-Render in publication paper?

I want to publish paper in journal but I have figures which have drawn with bio-renders, can I put them in the paper or which other software I can use to draw them?

27 February 2021 8,179 1 View

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Please. Could you help be in implementing Fractal Discrete Cosine Transform algorithm in Matlab?

I do need the Matlab code of Fractal Discrete Cosine Transform (FDCT). Can anyone who has already implement this code, help me with the implementation of this transform?

24 February 2021 5,602 2 View

Is it possible to set a breakpoint at a specific DYNAMIC instruction using the GNU Debugger (GDB)?

Using GDB, it is straightforward to debug and monitor a target program by setting a break-point at a specific instruction, since the instruction addresses are known. However, we have two...

24 February 2021 1,598 2 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

What is the most prevalent drug related problem in the developed countries?

Detecting the drug related problems is an important step in the pharmaceutical care plan

21 February 2021 5,421 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Alpha and dense granules in platelets not visible in Transmission Electron Microscopy?

I am trying to visualize alpha and dense granules in platelets. However, it seems like the granules were not stained well. Is there any way I can improve my staining technique to obtain better...

04 February 2021 5,925 9 View

What is the difference between human umbilical vein endothelial cells (HUVECs) and bone marrow endothelial cells?

Hello I am new to work on endothelial cells, and I have been reading about them a lot and trying to know the difference between human umbilical vein endothelial cells (HUVECs) and bone marrow...

28 January 2021 8,765 4 View

How to analize platelet titration results?

Hi, I am working with TRAP-stimulated and unstimulated platelets in PRP Right now I am titrating the antibody CD62P but I have several questions about how to analyze my data to select the best...

13 January 2021 8,991 3 View

I want to do a phospho flow analysis of the Jak2-Stat5, class ABL and Ras targets, which Phospho flow cytometry Antibody is recommended to use?

The assay is in leukemia cells.

28 December 2020 9,817 4 View

PDX leukemia in immunodeficient mice show regression after a time. Anyone knows what is going on?

I am performing PDX ALL in NSGS mice, but something curious happens: some leukemias develop very well , but after a time the hCD45+ cells show a huge regression while the mCD45+ cells turn back to...

16 December 2020 7,344 2 View

Extraction of viral DNA from human blood?

Hi, I am looking to detect CMV DNA in immunocompromised leukemia patients followed by subsequent end-point PCR. Can anyone suggests a suitable kit for extraction of CMV DNA from human blood...

13 December 2020 4,024 5 View

Could anyone suggest me what is the best way/method to isolate total RNA from the platelets?

I have tried to isolate platelet total RNA from the human and sheep blood using TRIzol reagent. After isolation of platelets from the blood samples, total RNA extracted, and quantified using...

02 December 2020 6,165 4 View

Buffy Coat preparation? What freezing media is best for ensuring optimal integrity?

Hello all. I am trying to isolate buffy coat from whole blood (sodium citrate), and planning on cyropreserving it for later analysis (~3-5 years). As to what analysis would include, we are leaving...

17 November 2020 1,899 3 View

Flow cytometry - why do we see decreasing flourescense signal on Navios Flow cytometer?

We have observed a decreasing median fluorescence intensity (MFI), and recently extracted data from seven years of analyses (se attached figure) showing a very clear decline in fluorescence...

12 November 2020 4,694 3 View

Nina Petkova

Pathologic hemapoiesis- monoclonal unlimited proliferation of early WBC precursors without differentiation, which supresses normal hemopoiesis.

Susan Moreton

it is not a stem cell disorder. Mutation occurs in the myeloid cell line only. Abnormal cells then take over marrow and suppress all normal haematopoeisis.

Ahmed Mahmoud Taha Khattab

Due to abnormal uncontroled proliferation of clonal malignant leukemia cells that overcome and depresses normal hematopoiesis. So, finally the peripheral blood becomes filled with immature leukemia cells which are counted by automated analysers in the WBC chamber and major depression of Hb and PLT counts