Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

How to minimise the risk of being abused the profile of potential participants tagged in a social media post?

Dear colleagues, I got this one in one project I did ethics review. It's great to hear about your thoughts/experiences. "Doing an open recruitment online (i.e., via Facebook) requires public...

22 February 2021 3,322 3 View

Any suggestion on self-efficacy scale ?

Dear researchers, I am planning on conducting a research on the relationship between Language learning strategies, by Oxford (1990) and self-efficacy. I have found Morgan-Jinks Student Efficacy...

22 January 2021 8,432 3 View

I need to apply a questionnaire from an article. What statistical analysis do I need to use?

Hello, I found a survey about attitude toward language in an article which I find fit to use it in my thesis. The survey has 30 questions, which were put into 5 factors through factor analysis...

21 January 2021 9,419 3 View

Why did I obtain abnormal staining by TUNEL assay?

I use Abcam TUNEL Assay Kit - HRP-DAB (ab206386) on mouse tissues. The staining showed a pattern that I expected to see with a specific layer of cells dying due to drug treatment. But the...

12 January 2021 144 1 View

Is there a generalized method to derive current-mode model of any DC-DC converters?

Hi all, As new DC-DC converters are being proposed, associated control method should be proposed and based on the small-model signal analysis of the DC-DC converters. Using average space state...

06 January 2021 7,247 3 View

Is it need to test Cronbach's Alpha for extracted features from data?

As Cronbach's Alpha is usually used for test the reliability of measurements given by questionnaires, however, I wonder that whether I use this test for features extracted from behavioral data. I...

26 December 2020 9,602 2 View

How is datasets good?

Can you show me some ways to generate benchmark datasets?

22 December 2020 362 4 View

How to calculate the Water Stress Index for a basin?

Water Stress Index (WSI) and Environmental water requirements (EWR). Thank you.

06 December 2020 904 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

How to calculate the crystallinity of cellulose film?

27 February 2021 2,753 4 View

Alpha and dense granules in platelets not visible in Transmission Electron Microscopy?

I am trying to visualize alpha and dense granules in platelets. However, it seems like the granules were not stained well. Is there any way I can improve my staining technique to obtain better...

04 February 2021 5,925 9 View

How to analize platelet titration results?

Hi, I am working with TRAP-stimulated and unstimulated platelets in PRP Right now I am titrating the antibody CD62P but I have several questions about how to analyze my data to select the best...

13 January 2021 8,991 3 View

How can we measure the surface relative content of deconvoluted Mn 2p3/2 XPS spectra?

Somewhere in papers, it is written as Mn 2p3/2 XPS spectra can be deconvoluted into four peaks at 640.83, 641.92, 642.97 and 644.56 eV assigned to Mn(II), Mn(III), Mn(IV), and the shakeup peak,...

24 December 2020 4,323 4 View

Could anyone suggest me what is the best way/method to isolate total RNA from the platelets?

I have tried to isolate platelet total RNA from the human and sheep blood using TRIzol reagent. After isolation of platelets from the blood samples, total RNA extracted, and quantified using...

02 December 2020 6,165 4 View

Buffy Coat preparation? What freezing media is best for ensuring optimal integrity?

Hello all. I am trying to isolate buffy coat from whole blood (sodium citrate), and planning on cyropreserving it for later analysis (~3-5 years). As to what analysis would include, we are leaving...

17 November 2020 1,899 3 View

Flow cytometry - why do we see decreasing flourescense signal on Navios Flow cytometer?

We have observed a decreasing median fluorescence intensity (MFI), and recently extracted data from seven years of analyses (se attached figure) showing a very clear decline in fluorescence...

12 November 2020 4,694 3 View

How to extract membrane protein of platelet ?

I would like to extract membrane proteins of platelets, but I can't find more detailed protocols. How should I extract without a commercial kit?

25 September 2020 1,165 3 View

How Can Fibrin Hydrogel Contraction/Compaction be Stopped?

Hi everyone, Currently I'm culturing cells in a 3 mg/ml fibrin hydrogel. However, the cells either destroy the gels (without any anti-fibrolytic compounds) or compact the gels when anti-fibrolytic...

01 August 2020 8,155 1 View