i cannot help down at that level of dna but I have amplified many samples where all of the stored dna was used from a storage tube and adding water to the empty tube dissolved enough dna from the dry tube sides to amplify very well for later pcr use but this is hugely more than one molecule but less than a measurable amount on nanodrop

Phi29 is suitable for amplification of DNA from single cell. Hovewer, it should be kept in mind, that such low input of template leads to a strong allele disbalance (i.e. amplification bias) during WGA.

May be you first measure the A260 of your sample, calculate the copy number using MW of a single cpy of the your DNA following the Avogadro Hypothesis and accordingly dilute your sample to a limit where it is expected to contain a single copy. Then you can do a Phi29 reaction of the dilutions to see up to which dilution it can amplify. 

I have not done this with Phi29, but we often do this with plasmid templates and PCR to estimate the detection limit of PCR r real time PCR assays. I hpe this strategy might also work with your template and Phi29.

Best wishes.'SD

Thank y'all ! Your comments are very helpful. I am going to go with a serial dilution. My lowest dilution is 0.0002 pg/uL. I will keep you posted on the success of WGA.

Cheers!

It will be interesting to hear how you get onSankalpi. You will need a specific test like pcr of a gene because the wga kits are prone to concatenation of the shortmer primers and incorporation of the ntps in random fashion when there is almost nothing to do so just measuring OD will always look like there is dna present but nested pcr hopefully will show good amplification. good luck

paul

Thanks Paul. Great input. I am planning on doing a gene/allele-specific PCR to test if I have actually amplified genomic DNA.

Cheers !