Has anyone been able to optimise DC-LAMP (LAMP3 / CD208) using immunohistochemistry in formalin-fixed biopsies?

Has anyone been able to optimise DC-LAMP (LAMP3 / CD208) using immunohistochemistry in formalin-fixed biopsies? We have tried several available antibodies for use in human biopsies, but none are...

24 February 2019 6,461 3 View

Excessive boiling of proteins for Western Blot?

HI all. I was preparing my samples for Western blot, and accidentally boiled my proteins at 100C for around 8 min instead of the intended 5 min. My proteins of interest did not show up on the...

01 February 2018 4,504 3 View

Can anyone recommend good antibodies for NF200, CGRP, or isolectin B4?

Will be completing immunofluorescence experiments in formalin fixed mouse spinal cord/DRG and wanted to know if anyone could recommend antibodies for NF200, CGRP or IB4 that they've used in the...

31 January 2018 9,500 4 View

Any advice on pressure distribution in fluent?

I need to determine the pressure distribution around a cylinder that is being subjected to external flow. How can I do this?

22 October 2015 2,278 1 View

Does anyone have experience working with any commercially available antibodies for TRPV1 that have worked well for you?

Or on the other hand, have you used a TRPV1 antibody that has been a failure?

21 September 2014 9,922 4 View

How can we minimise / avoid autofluorescence of Eosinophils in Hodgkin tissue?

We are performing multiplexed immunofluorescence on formalin-fixed, paraffin embedded Hodgkin lymphoma. A major issue has been autofluorescence or non-specific binding of what we think are...

19 November 2013 548 2 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

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02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View