Hello! So recently I have started with the isolation of adult cardiac myocytes from swiss albino mice, while the protocol is a standard one and I do initially get a decent percentage of rods (55-60%) but the rods quickly lose their shape and become circular within 24 hours ( to be precise somewhat 16-18 hours after culture they lose their rod appearance), can anyone help with this? Also I use Collagen coated plates for this culture and I'm also simultaneously facing poor cell adhesion issue with this culture.