Losing DNA during library preparation Nanopore?

24 July 2020 1 9K Report

Hi all,

I am starting my nanopore library preparation with 1.5 ug plasmid DNA, however after library preparation and loading on the flow cell, I notice that I am losing a lot of DNA and my sequencing run isn't using all pores.

Has anybody experienced this? and what is the maximum amount of input DNA that you may have used?

Kshitij Rai

Hi Lisa,

How many pores does the device say it's using during sequencing? You may know this already, but the MinION device sequences using only a max of 512 pores during each run. All the available pores will be scanned, and the best set of 512 pores (or slightly lesser) will be used. You can also recover a large fraction of the pores if you wash the flow cell using Nanopore's recommended flow cell wash kit.

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