Lisa Stein

5 Questions 4 Answers 0 Followers

Questions related from Lisa Stein

How to confirm I have a full natural plasmid?

Hi all, I've done WGS using nanopore, and have been able to successfully see 4 circularized plasmids and a full circularized chromosome. How do I know that these are the full plasmids without...

30 March 2023 8,967 2 View

Nanopore unclassified reads, how to resolve them?

Hi all, I recently did a nanopore run using the ligation sequencing kit with native barcoding. I had a high amount of reads and data generated. The biggest issue I have is that when I...

10 August 2022 2,990 3 View

Is it possible that a IncX and IncF plasmid fused?

I recently performed plasmid extraction and nanopore sequencing. I have found what looks like a plasmid with replicon types IncX and IncF. Even with spAdes, canu, unicycler (illumina and nanopore...

30 September 2020 7,933 2 View

Losing DNA during library preparation Nanopore?

Hi all, I am starting my nanopore library preparation with 1.5 ug plasmid DNA, however after library preparation and loading on the flow cell, I notice that I am losing a lot of DNA and my...

24 July 2020 9,427 1 View

Too many bands after digestion with restriction enzymes?

Hello Everybody, I am in the process of extracting a natural plasmid containing the CTX-M 1 gene. I do not know what plasmid (type/Inc etc) is present in my sample but I have been successful in...

29 May 2019 939 3 View