Are you interested to submit a paper in the special issue "Solar Radiation" in "Remote Sensing" ?

I you are interested to submit a paper in the special issue "Solar Radiation" in "Remote Sensing", please have a look on the 2 following files and/or on the web site :...

01 February 2021 2,954 3 View

Looking for Myc or Tags-T2A-PURO/Blast vectors?

Hi all, I am looking for a vectors backbone containing an expression cassette for epitope tagging my candidates are -Puro/blast-T2A-3MYC (other tag will do ) -MYC-T2A-PURO/Blast (other tag will...

29 September 2019 5,904 1 View

How to run seasonal trajectories using GUI HYSPLIT model

29 December 2016 9,477 9 View

To illustrate an exhibition devoted to insect bioluminescence, I am looking for living adults/larvae of Coleoptera Lampyridae. Can anyone help me ?

06 January 2015 2,403 6 View

Are there any Blast searches on HeLa genome?

Does anybody know how we can perform a blast searches of specific DNA sequence on the hela genome (and other specific cell lines). Many cell lines have been sequenced but it is not obvious these...

26 November 2014 2,634 3 View

Is anybody aware of a direct use of correspondence analysis in association rule learning (from pattern recognition & DB engineering view point)?

As the basic concepts used in association rule learning are related to conditional probability and ratio to independence, I was wondering if Correspondence Analysis has been used in the literature...

01 January 1970 5,480 1 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View