For example, oxazine type moieties which forms diastereomers, how the separation can be done? Chiral HPLC is the only possibility?
The separation of true diastereomers does NOT require the use of chiral HPLC columns. Please contact someone local with experience in chromatography to help you with your project.
For diastereoisomers, crystallization or regular chromatography should be fine. Enantiomers require chiral chromatography.
Diastereomers are typically separated by column chromatography using an appropriate solvent combination and are characterized by methods such as NMR spectroscopy and other analytical techniques. Once separated, recrystallization can be performed to obtain crystals suitable for X-ray crystallography.
In contrast, enantiomers require chiral chromatography for separation because they share identical physical properties in an achiral environment. However, when passed through a chiral column, they can be separated due to their interactions with the chiral stationary phase. Additionally, enantiomers can be physically separated through chiral resolution, which involves derivatizing the enantiomers with chiral derivatizing agents (chiral resolving agents) to form a pair of diastereomeric derivatives. These diastereomeric derivatives can then be separated by conventional methods.
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