Is there an Innovation in representation of the Taxonomy (flat, hierarchical, network, ...)?

Many research fields use Taxonomy for classification, labeling or grouping of different findings. Would like to get your argumentation about the future of Taxonomy! Best with references minimum 20...

24 February 2020 5,997 4 View

Are there any open-source meshing tools for structured curvilinear grids?

After starting my CFD life using meshless methods I am now expanding my horizon and I have started work on a code that works with structured curvilinear grids. The group that built the code is...

24 January 2015 3,103 13 View

LacZ/ccdB counter-selection - any thoughts on a 2 counter-selectors strategy?

updated (2014.07.24) I m constructing a DNA cassette (with genes sacB, SmR, and LacZ), which will replace the galK region of an E. coli strain lacking LacZ after recombination. 1) Anyone has a...

14 May 2014 7,217 2 View

Symbiosis evolution: in what range physiological "loss of traits" help symbiosis evolution

Literature about symbiosis acknowledge a genetically "loss of traits" is typical of symbiosis "evolution". (eg: genome reduction, gene loss) What can we say about physiological "loss of traits"...

19 June 2013 238 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

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02 March 2021 3,060 3 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View