Long (200+ bp) primer overhangs in In vitro transcription: Using primers to add poly A tail?

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In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

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Resistor definition in Piezoelectric bimorph with ANSYS workbench

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How do I increase the yield of my PCR product?

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Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

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Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

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How to calculate the annealing temperature of a primer pair?

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How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

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How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

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Frank R Burns

Hi John,

I can't think of a reason it won't start out OK, but you will be depleting the A base from your reaction mix faster than other nucleotides so you may shut down the reaction sooner than would happen without the long homopolymer.

Good Luck

Frank

Laurence Stuart Dawkins-Hall

With primers that long make sure they are PAGE purified and not simple desalted