John Yn

2 Questions 8 Answers 0 Followers

Questions related from John Yn

Long (200+ bp) primer overhangs in In vitro transcription: Using primers to add poly A tail?

I'm thinking of amplifying my DNA template (for downstream in vitro transcription [IVT]) using primers that have a long (200-250 bp) AAAA or TTTTT overhang, such that a poly A tail gets added...

02 February 2019 899 2 View

4 Extra Bands During Plasmid Linearization?

Hey everyone, I see my expected ~6 kb band in my gel after linearizing my plasmid (acquired from DH5alpha cells via a plasmid purification kit). However, I also see 4 extra 0.5-1 kb bands. The...

08 August 2018 4,034 2 View