Does anyone know at what concentration DSMZ supply bacterial DNA?

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Abhijeet Singh

I guess they provide the concentration of the DNA with the DNA sample.

Dirk Schmidt

Hello Eleanor,

about three years ago I ordered DNA from a specific Chlamydia trachomatis serovar that I wanted to use as positive control in qPCR.

On the tube that I got the DNA concentration was given in ng/µl. I diluted the DNA down to 1ng/µl which is always my standard concentration to start from and it worked well. I then diluted this DNA further to find the limit of detection.

We´ve been using this standard ever since for this diagnostic qPCR.

Best regards

Dirk