Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

How does the point & click method in SPSS form matching pairs in Propensity score methods?

What method does the SPSS point and click option run for propensity scores? That is does it do matching with replacement or matching without replacement, and does it do greedy or optimal...

17 March 2019 4,975 3 View

Has anyone tried to use Ag as an adhesive layer for thin film deposition?

I currently clean the SiO2 substrate with Piranha solution for 1-2 hours. I've also used Cr and Ti as the adhesive layer and neither have worked. Both samples create thin films that can easily...

02 October 2018 928 3 View

Does anyone know at what concentration DSMZ supply bacterial DNA?

13 June 2018 6,306 2 View

Where has this article been published online? Would you be willing to provide a URL please?

Please provide a link to the online article.

06 June 2018 5,587 2 View

Primary dendritic cell culture?

Hi could anyone point me in the direction of a paper that talks about the length of time that mature (i.e. not BMDC) DCs eg from lymph nodes can be incubated ex vivo before cell death?

12 November 2017 4,691 2 View

Can overlap extension PCR be used to simultaneously insert and remove base pairs?

I want to use overlap extension PCR to insert a domain into my construct. However I have just realised that upstream of the region where I want to insert the domain there are 19 amino acids...

22 May 2017 5,117 4 View

Can anyone advise on now to compare ligand binding sites in NMR and crystal structures?

New to crystallography...I want to compare ligand binding in a crystal structure to one generated using NMR. I have tried the pdbsum and the pisa servers but the hydrogen bonds that are identified...

27 December 2016 1,451 3 View

Does any one have any experience of creating a stable cell line using Calu-3 cells?

I am looking to create a stable cell line using Calu-3 cells. My initial plan is to co-transfect with pBABE-puro and select with puromycin. Does anyone have any similar experience with Calu-3? I...

16 August 2016 3,334 5 View

Do I need to codon optimise my sequence for expression in a mammalian cell line?

My POI is a human protein. However we when we had the DNA sequence synthesized we codon optimised for S.cerevisae (for protein expression). However, I now wish to do some interactome studies in a...

20 July 2016 1,429 3 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

Can you perform the Murexide test on samples fixed in Formalin?

I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples

22 February 2021 1,328 1 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

What is the best etching method for Mg-Ca alloy?

I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?

21 February 2021 2,558 5 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

What is the stability of 2 3-dihydroxyquinoxaline in acidic water?

I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...

20 February 2021 5,820 4 View