Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

How to estimate the extent to which the FE domain should be meshed with explicit elements in Explicit-Implicit Time integration procedure?

I am developing a MATLAB code for shock problem and want to divide the FE domain into explicit and implicit elements (region of domain at vicinity to shock is meshed into explicit elements and...

15 January 2021 1,968 2 View

How to adjust a covariate in meta-analysis?

Suppose I am meta-analyzing proportions using their logit transformations. We did a meta-regression of the covariates age, gender, and disease severity score in studies and get respective beta...

11 January 2021 9,409 4 View

Is it possible that first waiting time decreases and then after a certain point waiting time start increasing?

The sevice rate is fixed and the arrival rate is changing. As I am plotting the graph of arrival rate with respect to waiting time in queue.

22 December 2020 2,455 1 View

Which gas is used for Bunsen burner in ASTM D6413 Vertical Flame Test?

I'm trying to make a Vertical Flame Test apparatus for flammability test of PVC based leather. I'm not sure about the type of gas which is to be used for generating the flame using bunsen burner.

09 November 2020 1,808 1 View

Excel formula for assigning grade?

I have a excel sheet with positive and negative value. I have 4 grades to be assigned. How can I use a formula to assign the grades? I have tried using the IF nested function but due to negative...

21 October 2020 5,648 7 View

How does culture impact our ability to learn?

I am working this area, please inform some web resource that will help me.

14 October 2020 9,107 7 View

How to produce ROC/AUC curve using various groundwater vulnerability method for comparative analysis?

ROC/AUC Vulnerability Groundwater Machine learning

11 October 2020 9,186 3 View

When to add H2DCFDA dye before or after treatment and why?

When to add ROS generation binding dye H2DCFDA, before or after treatment and why?

07 September 2020 4,962 3 View

Statistical test for Inter and intra rater agreement between 2 observers for gradings? any excel based template for the same?

Hello, I wish to calculate inter and intra rater agreement between 2 observers who are gradings Xrays for degree of bony fusion into 6 grades. In all there are 1750 xrays. Which statistical test...

11 August 2020 1,001 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View