Nirmal Verma

4 Questions 5 Answers 0 Followers

Questions related from Nirmal Verma

What i see in my primary neuron culture?

I see small aggregation-like particles in primary neuron culture from the hippocampi of rats. Can anyone suggest what are these ? And can we go with drug or other treatment assays with these...

09 September 2021 197 3 View

What is good protocol for fixation of rat Kidney and eye balls?

Does anybody know good protocol for rat kidney and eyeball fixation? I have frozen kidney and eyeballs in -80 and want to fix them for IHC. I tried few protocols but eyeball get broken or...

21 April 2017 6,111 3 View

Can we isolate gDNA from -80 stored brain homogenates (no tissues)?

I have brain homogenates (supernatant after centrifugation) stored in -80 long ago. I need to do genotyping in those samples but i do not have blood of those samples. What i have is homogenates...

14 September 2016 2,866 3 View

Why Immuno-precipitation of a protein from plasma samples gives high background in western blot ?

I Co-Immuno-precipitated proteins from plasma sample and did WB for another protein supposed to be conjugated with first. I found high background in WB. I found this is due to plasma samples, Is...

23 June 2016 8,280 9 View