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Questions related from Nirmal Verma
I see small aggregation-like particles in primary neuron culture from the hippocampi of rats. Can anyone suggest what are these ? And can we go with drug or other treatment assays with these...
09 September 2021 203 3 View
I loaded equal amount (100ug) of red blood cell lysate, WBC lysate, pancreas lysate and plasma samples on same western gel. I need to show equal loading of samples. How i can show that? I tried...
03 June 2019 1,691 0 View
Does anybody know good protocol for rat kidney and eyeball fixation? I have frozen kidney and eyeballs in -80 and want to fix them for IHC. I tried few protocols but eyeball get broken or...
21 April 2017 6,193 3 View
Is Peroxidase kit (Biotinylated secondary antibody followed by ABC reagent incubation) used in IHC light sensitive? Means will it show reduce signal after DAB if we exposed section to light during...
14 April 2017 1,527 3 View
I have brain homogenates (supernatant after centrifugation) stored in -80 long ago. I need to do genotyping in those samples but i do not have blood of those samples. What i have is homogenates...
14 September 2016 2,888 3 View
I Co-Immuno-precipitated proteins from plasma sample and did WB for another protein supposed to be conjugated with first. I found high background in WB. I found this is due to plasma samples, Is...
23 June 2016 8,297 9 View
I want to prepare amyloid stock solution in DMSO. Article says sonication for 15 minutes at RT. they not mentioned intensity, I am confused, as this much long may be warm solution too much.
06 June 2016 8,976 4 View
What dilution factor is good for Alexa 488 conjugated secondary antibody from invitrogen? I used 1:300 and getting background and autofluorescence. Is there any dilution factor that could be use...
17 April 2015 1,676 11 View
