We did for the first time the CCl4 liver inflammatory model in mice. Note that we did not start with phenobarbital previous to CCl4 protocol and we gave intraperitoneally 2ml/Kg (2:5 v/v CCl4 in mineral oil) that we planned to do twice a week. After the first injection the animal facility people told us that the mice suffered (abdominal pain and that they walked abnormally) and the next day the disconfort seemed less but there is still some appaerence of pain, although they have to admit much less. The animal facility people want to do something about it, before the next injection this Friday. Put analgesia.
Can someone tell us if this pain is normal in mice? Do you think that it is possible that our solution was corrupted somehow?
Finally is it OK to add a pain killer to the mice in this model? As many should give liver inflamation by themselves we are reluctant to accept this change? if yes which one is suggested for this type of experiments?
We did not notice this withing the first week. We injected i.p. twice a week.
We also did not notice any prolonged pain within our study when injecting CCl4 IP. With the initial dose of CCl4, there could be an acute inflammatory response that is potentially lethal for the mouse, but this response is highly strain dependent. Overall, the side effects of the initial CCl4 administration often subsides within 48 hours after the first dose.
Also, it is very important to keep the animal properly restrained and tilted when performing an intraperitoneal injection. These practices will help avoid traumatizing the organs when inserting the needle (which should be inserted at about a 30 degree angle).
Hi, I was giving ip injections of CCL4 to C57Bl6 mice and also saw that after injection they showed signs of abdominal pain and disconfort that lasted 24h and often they lost weight during that period (a sign of disconfort).
I hope I helped you
Marta
Dear Jean,
we used Balb/cJ mice for induction of chronic liver damage plus inflammation and we used CCl4 too for 12 weeks. We injected mice ip twice a week with 1 ul/g in a mixture 1:7 v/v of CCl4 in olive oil. We did not notice any sign of disconfort. We monitored the weight of animals before any injection and we did not observe any weight loss throughout the treatment. Pay attention to the amount of oil injected each time and perform the injection carefully and not too fast. Anyway the use of enrichments in animals cages (I mean cardboard tunnel et al) deeply improved their status. Have a good day! Marika
Dear Jean,
I am currently injection CCl4 by IP and I also noticed exactly all the feature which you described above, I used 0,5ul/1 gm weight (1:3 in mineral oil). I also noticed drop in body weight once. Did you come up with any solution to avoid this pain?
Best regards
Umesh
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