Marika Crescenzi

7 Questions 15 Answers 0 Followers

Questions related from Marika Crescenzi

Parallelism between mRNA and protein changes: which is the best approach?

Hello! I'm evaluating which is the best approach to observe protein levels changes after having observed for the same target mRNA level changes. Preliminary data with the same time point of...

10 June 2022 6,766 0 View

Which is the recommended Mounting medium after Oil red staining in frozen sections?

Dear all, Which is the best mounting medium for liver frozen sections stained with oil red O? An aqueous one as Ab104135 could be the solution (even if suggested for IF)? Thanks in advance.

17 March 2022 2,606 3 View

Is it possible to use expired gelatin powder for flask coating?

Hi everyone. Has anyone some experience with expired powder gelatin to be used for flask coating? Thank so much. Marika

21 February 2022 9,508 3 View

Tissue Homogenates stored in Trizol at -80°C?

Dear all, is there anyone which experienced to homogenate tissues in trizol, but did not proceed immediately with RNA extraction? I mean, is it possible to homogenate in trizol and store your...

13 April 2017 6,306 3 View

How many times can I re-use an aliquot of Bouin's solution?

In the standard procedure for Masson's trichrome staining I have to pre-heat Bouin's solution at 56°C for 15'. My question is: how many times can I re-use an aliquote of pre-heated Bouin's...

30 September 2014 6,795 15 View

Could anyone give me a detailed protocol for exosomes-conjugation to ALDEHYDE/SULFATE latex beads for cytofluorimetric analysis?

24 September 2013 7,621 2 View

Exosome Isolation from body fluids?

In evaluating commercially available kits for exosome isolation, the main question is: can I consider wound fluids/ascites' composition/characteristics similar to serum? I mean, for those kits...

18 March 2013 3,372 4 View