One of the main differences is on p53 expression. In this way, HepG2 cells carry wild-type p53, whereas Hep3B and Huh7 cells have null and point mutations at p53 codon 220 respectively. 

This paper will be of great use for you : https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3061558/. 

Good luck. 

Wouter

You should be aware that all of these cell lines are transformed (cancerous) cells and, as such, may differ significantly in their metabolic profile. For example, the HepG2 cell line is widely reported not to metabolize alcohol via cytochrome p450 2E1 or alcohol dehydrogenase, and derivatives of this cell line that express either or both of these enzymes have been created and used extensively to study alcohol metabolism. I am sure similar metabolic profiles have been reported for the other lines that you identify. Similarly, all of these cell lines show a propensity for 2 dimensional culture and rapid cell division, something that does not readily happen in hepatocytes in vivo - this may be particularly relevant depending on the type of drug you are studying. In addition to the point raised about p53, you should also be aware the Hep3B cell line expresses the hepatitis B virus and should be treated with appropriate bio-hazard precautions,

In short, I think the suitability of each of these cell lines for pharmacokinetics may depend specifically on the drug type you are looking to study. Similarly, hepatotoxicity can extend beyond the specific effect of a drug on hepatocytes and may require an in vivo model to accurately mirror what may happen in humans.

Dear Iskander and Iain,

Thank you both for your quick and useful response!

Wouter

Hello

yes , these the important differences between them 

Good Luck

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3061558/

http://file.scirp.org/pdf/JCT_2013042314042157.pdf

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0072252

http://jvi.asm.org/content/77/22/11875.full

We just identified that Hep3B has p53-FXR2 fusion due to 76 kb focal deletion within chromosome-17 in this paper

Mutant p53s and chromosome 19 microRNA cluster overexpression regulate cancer testis antigen expression and cellular transformation in hepatocellular carcinoma | Scientific Reports (nature.com)