Hello Angeles Estelles

I have not used Lipofectamine 3000 in mice, nevertheless you can refer to the biosafety evaluation section of the research paper attached. It will be helpful.

Biosafety Evaluation

To evaluate the biocompatibility of PSP@MB, histological examination of hematoxylin and eosin (H&E) staining of vital organ (heart, liver, spleen, lung, kidney) sections were detected. The result of H&E staining assay showed symptoms of swell, fatty and vacuolar in the liver cells treated by lipofectamine 3000. The PSP@MB+US group demonstrated the normal morphology of liver cells compared with plasmid group. Two pathologists confirmed the histopathological diagnosis of diffuse hepatic edema in lipofectamine 3000 group. The histopathological results demonstrated PSP@MB had excellent biocompatibility, but lipofectamine 3000 had abnormal liver damage at a certain concentration in vivo.

The liver functions (ALT and AST), the renal functions (Crea), Direct Bilirubin (D BILI) and routine blood parameters (hemoglobin and white blood cell) were tested. All the biochemical parameters in serum and routine blood test remained at a normal range in PSP@MB and ultrasound group. Compared with PSP@MB and ultrasound group, the activity of ALT, AST and D BILI were increased significantly in lipofectamine 3000 group.

Reference:

Article Echogenic PEGylated PEI-Loaded Microbubble As Efficient Gene...

Regards.

Thank you very much Malcolm Nobre . This is very useful

Low toxicity and can transfect primary cells from mice. Much better performance for cell lines. need to optimize the condition. Can not keep more than 6-8 hrs.