15 October 2015 13 5K Report

Dear All,

In my experiment, I need cell to be 50% confluent in 100mm dish to treat drug and collect samples after 24h.

To prepare cell test group (I use large number of cells. 8 of 100mmdish), Which is better between:

(1) subculture cell from the same one flask into mutiple vessel equally, and let grow to wanted 50% confluent, then treat the cells.

(2) subculture cell from the same one flask to multiple flask first. Let all flask grow, then collect all cells mixing together into single solution, count cells, then subculture 50% confluent cell number into each new dish, equally. Then after 24h (lag phase, no cell number change) treat the cells.

The idea of first method is for eaiser to distribute small cell number for preparing many group of large number of cell, but not sure whether cell grow/become more difference (in cell number between each group) in longer time. While, the idea of 2nd method is for control cell number, because the cell is in lag phase (after passage 24hr) and less likely to grow more from what it has been passaged.

Should these two method be the same?

Thank you

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