are there any problems may result from leaving fish tissues for long periods in 10% neutral buffered formalin?
the enzyme activity was calculated as U/ml, i want to divide it on the total protein concentration which calculated as mg/g for calculation of the specific enzyme activity as U/mg protein.
31 December 2017 4,806 6 View
iam not specialized in histopathology and have some work in this area so, iam in need to identify and diagnose the pathological changes involved in my tissues. so, iam searching for a software...
04 May 2017 2,596 6 View
I want to publish my research paper in any reputed journal within short period not exceed two months. . Are there any free journals available to publish withinn 2 months?
11 December 2016 3,420 7 View
i want to stain the cell protein , so what is the proper stain of it?
10 November 2016 6,346 0 View
i want to know the latest list of highest impact factor journals thanks in advance
06 July 2016 6,597 28 View
i used neutral buffered formalin to fix fish tissues liver , muscles and gills is it important to make three changes from it before put these samples in 70% ethyl alchol? are there in changes for...
05 June 2016 5,002 8 View
i reserved fish tissues in the deep freezer for a period of time for later fixation and doing histopathology i don't know if this step is wrong or no ? first :- samples were transported to the...
04 May 2016 7,425 9 View
i made total lipid assay in fish muscle tissue using kits for serum the unit of concentration for serum total lipid is mg/dl what is the unit in the case of tissue?
04 May 2016 2,715 0 View
iam in need to know what is the best fixative and its preparation protocol to fix fish muscles and liver for latter histopathology ?
04 May 2016 6,855 9 View
I am going to work on heavy metal estimation in various fish organs and studying the effect of these metals on fish physiology.
02 March 2015 997 11 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...
05 August 2024 853 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...
03 August 2024 9,918 0 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...
31 July 2024 5,645 2 View