How can i convert u/ml to u/mg?

the enzyme activity was calculated as U/ml, i want to divide it on the total protein concentration which calculated as mg/g for calculation of the specific enzyme activity as U/mg protein.

31 December 2017 4,745 6 View

Are there any histopathology image analysis softwares?

04 May 2017 2,540 6 View

Are there any free reputed journals available to publish within short period?

11 December 2016 3,368 7 View

What is the proper stain for protein???

i want to stain the cell protein , so what is the proper stain of it?

10 November 2016 6,286 0 View

What is the latest list of highest impact factor journals?

06 July 2016 6,497 28 View

Is it important to change 10 % neutral buffered formalin ?

05 June 2016 4,934 8 View

Are there any problems if i reserved fish tissues in deep freezer at -20 for later histopathology?

04 May 2016 7,360 9 View

What is the unit of concentration of total lipid assay in fish tissues?

i made total lipid assay in fish muscle tissue using kits for serum the unit of concentration for serum total lipid is mg/dl what is the unit in the case of tissue?

04 May 2016 2,650 0 View

What is the best fixative for fish tissue fixation ?

04 May 2016 6,791 9 View

Can anyone suggest an ideal method for heavy metal estimation of fish organs?

I am going to work on heavy metal estimation in various fish organs and studying the effect of these metals on fish physiology.

02 March 2015 939 11 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View