i made total lipid assay in fish muscle tissue using kits for serum the unit of concentration for serum total lipid is mg/dl what is the unit in the case of tissue?
are there any problems may result from leaving fish tissues for long periods in 10% neutral buffered formalin?
01 February 2018 5,400 0 View
the enzyme activity was calculated as U/ml, i want to divide it on the total protein concentration which calculated as mg/g for calculation of the specific enzyme activity as U/mg protein.
31 December 2017 4,806 6 View
iam not specialized in histopathology and have some work in this area so, iam in need to identify and diagnose the pathological changes involved in my tissues. so, iam searching for a software...
04 May 2017 2,596 6 View
I want to publish my research paper in any reputed journal within short period not exceed two months. . Are there any free journals available to publish withinn 2 months?
11 December 2016 3,420 7 View
i want to stain the cell protein , so what is the proper stain of it?
10 November 2016 6,346 0 View
i want to know the latest list of highest impact factor journals thanks in advance
06 July 2016 6,597 28 View
i used neutral buffered formalin to fix fish tissues liver , muscles and gills is it important to make three changes from it before put these samples in 70% ethyl alchol? are there in changes for...
05 June 2016 5,002 8 View
i reserved fish tissues in the deep freezer for a period of time for later fixation and doing histopathology i don't know if this step is wrong or no ? first :- samples were transported to the...
04 May 2016 7,425 9 View
iam in need to know what is the best fixative and its preparation protocol to fix fish muscles and liver for latter histopathology ?
04 May 2016 6,855 9 View
I am going to work on heavy metal estimation in various fish organs and studying the effect of these metals on fish physiology.
02 March 2015 997 11 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...
05 August 2024 853 3 View
When the entire neocortex is ablated in rodents, although they are still able to swim, all the limbs move continuously and asynchronously (Vanderwolf 2006; Vanderwolf et al. 1978). Normal animals...
03 August 2024 835 3 View
I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....
31 July 2024 3,756 0 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
What is the thermal expansion coefficient of human muscle?
27 July 2024 7,190 0 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
I am looking for a lipid waste disposal method, keeping in mind the environmental, health and safety aspect of lipid waste. Could someone please provide guidelines or the lowest acceptable...
25 July 2024 637 5 View