Leaving fish tissues in 10% neutral buffered formalin fixative for long period can cause any problems ?

are there any problems may result from leaving fish tissues for long periods in 10% neutral buffered formalin?

01 February 2018 5,347 0 View

How can i convert u/ml to u/mg?

the enzyme activity was calculated as U/ml, i want to divide it on the total protein concentration which calculated as mg/g for calculation of the specific enzyme activity as U/mg protein.

31 December 2017 4,745 6 View

Are there any histopathology image analysis softwares?

04 May 2017 2,540 6 View

Are there any free reputed journals available to publish within short period?

11 December 2016 3,368 7 View

What is the proper stain for protein???

i want to stain the cell protein , so what is the proper stain of it?

10 November 2016 6,286 0 View

What is the latest list of highest impact factor journals?

06 July 2016 6,497 28 View

Is it important to change 10 % neutral buffered formalin ?

05 June 2016 4,934 8 View

Are there any problems if i reserved fish tissues in deep freezer at -20 for later histopathology?

04 May 2016 7,360 9 View

What is the best fixative for fish tissue fixation ?

04 May 2016 6,791 9 View

Can anyone suggest an ideal method for heavy metal estimation of fish organs?

I am going to work on heavy metal estimation in various fish organs and studying the effect of these metals on fish physiology.

02 March 2015 939 11 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View