Leaving fish tissues in 10% neutral buffered formalin fixative for long period can cause any problems ?

are there any problems may result from leaving fish tissues for long periods in 10% neutral buffered formalin?

01 February 2018 5,347 0 View

How can i convert u/ml to u/mg?

the enzyme activity was calculated as U/ml, i want to divide it on the total protein concentration which calculated as mg/g for calculation of the specific enzyme activity as U/mg protein.

31 December 2017 4,745 6 View

Are there any histopathology image analysis softwares?

04 May 2017 2,540 6 View

Are there any free reputed journals available to publish within short period?

11 December 2016 3,368 7 View

What is the latest list of highest impact factor journals?

06 July 2016 6,497 28 View

Is it important to change 10 % neutral buffered formalin ?

05 June 2016 4,934 8 View

Are there any problems if i reserved fish tissues in deep freezer at -20 for later histopathology?

04 May 2016 7,360 9 View

What is the unit of concentration of total lipid assay in fish tissues?

i made total lipid assay in fish muscle tissue using kits for serum the unit of concentration for serum total lipid is mg/dl what is the unit in the case of tissue?

04 May 2016 2,650 0 View

What is the best fixative for fish tissue fixation ?

04 May 2016 6,791 9 View

Can anyone suggest an ideal method for heavy metal estimation of fish organs?

I am going to work on heavy metal estimation in various fish organs and studying the effect of these metals on fish physiology.

02 March 2015 939 11 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View