i want to stain the cell protein , so what is the proper stain of it?
are there any problems may result from leaving fish tissues for long periods in 10% neutral buffered formalin?
01 February 2018 5,400 0 View
the enzyme activity was calculated as U/ml, i want to divide it on the total protein concentration which calculated as mg/g for calculation of the specific enzyme activity as U/mg protein.
31 December 2017 4,806 6 View
iam not specialized in histopathology and have some work in this area so, iam in need to identify and diagnose the pathological changes involved in my tissues. so, iam searching for a software...
04 May 2017 2,596 6 View
I want to publish my research paper in any reputed journal within short period not exceed two months. . Are there any free journals available to publish withinn 2 months?
11 December 2016 3,420 7 View
i want to know the latest list of highest impact factor journals thanks in advance
06 July 2016 6,597 28 View
i used neutral buffered formalin to fix fish tissues liver , muscles and gills is it important to make three changes from it before put these samples in 70% ethyl alchol? are there in changes for...
05 June 2016 5,002 8 View
i reserved fish tissues in the deep freezer for a period of time for later fixation and doing histopathology i don't know if this step is wrong or no ? first :- samples were transported to the...
04 May 2016 7,425 9 View
i made total lipid assay in fish muscle tissue using kits for serum the unit of concentration for serum total lipid is mg/dl what is the unit in the case of tissue?
04 May 2016 2,715 0 View
iam in need to know what is the best fixative and its preparation protocol to fix fish muscles and liver for latter histopathology ?
04 May 2016 6,855 9 View
I am going to work on heavy metal estimation in various fish organs and studying the effect of these metals on fish physiology.
02 March 2015 997 11 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
