What are the basic features for a oxygen atmosphere controlled furnace ? Materials of heating elements? it is mandatory that it be a tubular furnace?

Im starting a research in telurite glasses doped with metallic oxides (Au and Ag) and I need to buy a furnace. Since I dont have much budget, I need a basic furnace configuration to oxidize...

09 January 2020 7,790 1 View

How can i know if my cell type expresses a certain type of enzyme?

In an informatic database, like typing the name of the enzyme and then the information of the enzymes expressed in that particular cell type.

16 May 2019 2,893 3 View

Any experts in Yeast?

Hello, I am looking for somebody with knowledge about S. cerevisiae yeast and petit colonies. Cheers, Estefania

19 August 2018 8,138 5 View

How to extract Cryptosporidium DNA from stool samples?

Does anybody know of a basic technique to extract protozoan DNA from stool samples? Thanks

22 February 2018 4,514 2 View

Can you use a pair of primers, used for Taqman qPCR, in SYBR qPCR without probe?

I am trying to use a qPCR for diagnostic of a phytopathogen. I have a pair of primers, designed and used with a probe in a Taqman qPCR for detection and diagnostic. I got the pair of primers but...

10 January 2017 8,351 3 View

Anyone interested in a joint study on Crenosoma species?

16 November 2016 3,131 3 View

How can I define a catalytic cracking kinetic model as an UDF Ansys Fluent?

08 September 2016 2,719 3 View

Has anybody used MOODLE on mobile devices?

Would you recommend this as an option for online training?

02 March 2016 3,262 4 View

How can I perform the interaction of an explicit solvent ( atomic ) with wall fixed temperature using an implicit wall?

Hi all, someone knows a trick in molecular dynamics for contacting a fluid (solvent explicit ) with a wall fixed temperature but using an implicit wall ( idealized impenetrable wall that interacts...

08 September 2015 3,451 2 View

How can I define a new material in Fluent?

Dear all, I am trying to define many new materials in Fluent: Drygas formed by hydrogen, methane, and ethane; and LPG formed by propane and n-butane. Can I define these new materials just...

21 August 2015 7,003 6 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View