IVT synthetic design

01 January 1970 0 5K Report

In the design of DNA sequence for the production of synthetic mRNA(IVT), should the sequence of 5' and 3' UTRs be antiparallel?

Badges
Science topic

More Zarin Sharifnia's questions See All

How to Define a new component in aspen plus ?

Hi I am trying to define an inorganic solid component, tetra calcium aluminoferrite, available in clinker produced in the cement industry. Unfortunately, I couldn't find it in the data bank. I...

03 December 2023 9,915 0 View

Can Anyone help me regarding the source of simulation tool CH4MOD for methane estimation?

CH4MOD is a semiempirical model for estimation of methane emission.

26 November 2021 9,350 0 View

The COD is increased in the experiment rather then decreasing?

hello sir, during my experiment the COD of the paper and pulp mill effluent (black liquor) is increased rather then decreasing after treatment with bacterial isolates. pls help me out

16 December 2018 455 1 View

Mouse Optic nerve crush injury (Survival surgery)?

I need suggestions on performing optic nerve crush in mice. It has been challenging to expose an optic nerve. I appreciate if you can share your experiences. Thank you!

22 May 2018 9,328 1 View

Why does the cumulative metabolic gas production from a bactch culture of bacteria over time follow a sigmoidal curve?

Sigmoidal curves are used to describe the population kinetics of microbial growth on a logarithmic scale. Many studies show that cumulative production of metabolic gases over time also follow a...

08 April 2016 9,315 1 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Determining the worth of a point improvement in Hamilton Depression Scale?

Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...

05 August 2024 3,246 1 View

The Optimal Experimental Approach for Gene Function Analysis?

What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...

04 August 2024 6,265 1 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

IHC profiler in image j software. has anyone used it to quantify nuclear DAB positivity?

Dear researchers. I tried using the IHC PROFILER in image j to quantify nuclear DAB staining. I followed the instructions in the original article by "Varghese F, Bukhari AB, Malhotra R, De A...

29 July 2024 2,229 0 View