The CyaA reporter assays is a classical method to determine if a bacterial effector is translocated to a host cell, commonly epithelial cell lines. In principle, this technique should work too in other eukaryotic cells, but I do not know if it needs modifications to be used in amoebas, such as Dictyostelium or the same commercial kits for cAMP detection are useful.

Thank you a lot for your help.