I'm having an issue with differentiaon media (DM) of MC3T3-E1 Subclone 4 cells I am following the ATCC recommendations for media and supplements (alpha-MEM + 10% FBS + 1X anti/anti (Pen/strep/amphotericinB)) and culture conditions for revival.
I use using DM composition- 10mM BGP; 100ug/ml L-ascorbic acid; 10uM Dexamethasone, but there is debris formation and cells are not looking healthy
Any suggestions as to what might be the problem or recommendations for the same?
Below I have attached the pic of cells after DM 4days
10 uM concentration of Dex is very high and may result in toxicity. Usually 10-100 nM Dex is used.
Thank you for your response. I had also well not treated at all with Dexamethasone but cells still look the same.
Yes. Even if I have reduced the Dexa concentration. There is debris. Any reason?
Please check your control cells if they are healthy.
The 100 nm concentration of Dex alongwith ascorbic acid and beta glycerophosphate works fine.
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