Dear Pandey,

Please have a look at these links.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3964247/

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4752947/

Good luck

Thank u sir

In my opinion ..There is two methods..Either you  sliced thin section of tubes and kept on the plates for incubation or second, you  crushed the tubers and made dilution for the isolation process in both the conditions, sample must be surface sterilized.

Dear Sir Ajay Kumar Thank u for your valuable suggestion....actually i want know is there any specific method for isolation of endophytic bacteria from tuber crop to avoid epiphytic contamination

Dear Pandey,

You can go through the publication entited "Isolation and identification of endophytic bacteria from root tissues of Salvia miltiorrhiza Bge. and determination of their bioactivities"

Article in Annals of Microbiology 63(4) · December 2013

DOI: 10.1007/s13213-013-0614-0.

Thank u sir

After surface sterilization, plant material must be incubated in nutrient broth to check any epiphytic growth. If no growth occurs after 1 or 2 days, take out the plant material and after teasing, the inner tissues is used  to inoculate on any media t o get an endophyte..