Hello all,

I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria monocytogenes plasmids. Therefore I am planning an experiment that would include a constitutively expressed asRNA (complementary to the first 20 or so base pairs of the ncRNA) and assessing for growth phenotype in a myriad of conditions. My question is, is this a viable alternative to me developing a deletion system for L. monocytogenes plasmids? And is there anything that I should be wary of in using an asRNA knockdown mutant?

Thank you