I wanted to ask if anyone have ever performed isolation of differentiated adipocytes either from iPSC or MSC using methods other than FACS? Asking because it's not a really viable option for us, and wondering if there are any other methods that are available. I tried looking around for possible isolation methods, but other than FACS, the most probable would be density gradient centrifugation, but could not find any studies that have done this before.
Primary Adipocyte Protocols: Adapt methods from adipose tissue digestion (e.g., collagenase digestion + floatation).
Recent Studies: Search for terms like "adipocyte isolation without FACS" or "primary adipocyte centrifugation" for iPSC/MSC adaptations. Example: [Scientific Reports] (2020) used buoyancy to isolate adipocytes from 3D cultures.
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